Disclosures: Yuling Zhang, MD, PhD: No financial relationships or conflicts of interest
Objective: Metabolomics provides a powerful approach to discover potential biomarkers by analyzing the changes in metabolite profiling in real time. In this study, the primary objective was to conduct a targeted metabolomic study to examine the association between serum metabolites levels and post-stroke fatigue (PSF).
Design: A retrospective clinical pilot study. Setting : Academic acute inpatient rehabilitation facility. Participants : Forty-six acute inpatient rehabilitation patients were categorized as having either none/mild fatigue (n=22) or moderate/severe fatigue (n=24) based on their responses to the 2-item Fatigue Pictogram.
Interventions: All subjects received routine physical, occupational and speech-language pathology therapy.
Main Outcome Measures: Fatigue was measured with 2-item Fatigue Pictogram (FP). Primary outcome was fatigue score. Secondary outcomes were attention and memory, length of stay, discharge destination. Clinical characteristic data including liver and kidney function, white blood cell counts, hematocrit, body mass index and pain were recorded. A total of 102 serum metabolites levels were measured using ultra high performance liquid chromatography and mass spectrometry. The normalized data were analyzed using multivariate analysis, principal component analysis (PCA) and partial least-squares discriminant analysis (PLSDA) through MetaboAnalyst.
Results: This cohort has 83% of ischemic stroke and 17% of hemorrhagic stroke with a mean age of 69.7 ± 11.3 years. Fifty-two% of subjects reported moderate to severe fatigue (n=24). Grouping by post-stroke fatigue severity, there was a significant difference of methionine_sulfoxide level between none/mild fatigue group and moderate/severe fatigue group (FDR adjusted p < 0.05, 8.25±3.05 VS 11.73±5.03). Serum methionine_sulfoxide level predicted post stroke fatigue (AUC=0.720; p < 0.05). Conclusions: Our preliminary findings suggest that the level of serum methionine_sulfoxide has predictive value for the post stroke fatigue during acute inpatient rehabilitation, providing insight in mechanism of PSF and potential target for therapeutic intervention.
Level of Evidence: Level III
To cite this abstract in AMA style:
Zhang Y, Li Q, Chou RC, Wood L, Luo X, Wang Q. Pilot Study, Serum Metabolites Associated with Post Stroke Fatigue in Acute Phase [abstract]. PM R. 2020; 12(S1)(suppl 1). https://pmrjabstracts.org/abstract/pilot-study-serum-metabolites-associated-with-post-stroke-fatigue-in-acute-phase/. Accessed May 16, 2025.« Back to AAPM&R Annual Assembly 2020
PM&R Meeting Abstracts - https://pmrjabstracts.org/abstract/pilot-study-serum-metabolites-associated-with-post-stroke-fatigue-in-acute-phase/